HEMATOPOIESIS AND STEM CELLS Derivation of functional mature neutrophils from human embryonic stem cells

نویسندگان

  • Yasuhisa Yokoyama
  • Takahiro Suzuki
  • Mamiko Sakata-Yanagimoto
  • Keiki Kumano
  • Katsumi Higashi
  • Tsuyoshi Takato
  • Mineo Kurokawa
  • Seishi Ogawa
  • Shigeru Chiba
چکیده

1Department of Cell Therapy and Transplantation Medicine, University of Tokyo Hospital, Tokyo; 2Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, Tokyo; 3Department of Clinical and Experimental Hematology, University of Tsukuba, Ibaraki; 4Divison of Tissue Engineering, University of Tokyo Hospital, Tokyo; 5Department of Clinical Hematology, School of Health Sciences, Kyorin University, Tokyo; and 6The 21st Century COE Program, Graduate School of Medicine, University of Tokyo, Tokyo, Japan

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Hematopoietic differentiation of embryonic stem cells.

This review is focused on methods that are used to derive hematopoietic cells from embryonic stem cells (ESCs). One of the strategies that have been recently used to achieve this goal is an approach of mimicking the hematopoietic niche in vitro by using hematopoiesis-supportive feeder cells, cocktails of soluble hematopoietic growth factors and a variety of matrices. While there is clear eviden...

متن کامل

Lost in translation: pluripotent stem cell-derived hematopoiesis

Pluripotent stem cells (PSCs) such as embryonic stem cells or induced pluripotent stem cells represent a promising cell type to gain novel insights into human biology. Understanding the differentiation process of PSCs in vitro may allow for the identification of cell extrinsic/intrinsic factors, driving the specification process toward all cell types of the three germ layers, which may be simil...

متن کامل

HEMATOPOIESIS AND STEM CELLS Differences in lymphocyte developmental potential between human embryonic stem cell and umbilical cord blood–derived hematopoietic progenitor cells

Hematopoietic progenitor cells derived from human embryonic stem cells (hESCs) develop into diverse mature hematopoietic lineages, including lymphocytes. Whereas functional natural killer (NK) cells can be efficiently generated in vitro from hESC-derived CD34 cells, studies of Tand B-cell development from hESCs have been much more limited. Here, we demonstrate that despite expressing functional...

متن کامل

Development of osteoclasts from embryonic stem cells through a pathway that is c-fms but not c-kit dependent.

Osteoclasts are hematopoietic cells essential for bone resorption. To study the derivation of these interesting cells, we developed a stepwise culture system where stromal cells promote embryonic stem (ES) cells to differentiate into mature osteoclasts. Three phases to this differentiation process include (1) induction of hematopoiesis, along with the generation of osteoclast precursors, (2) ex...

متن کامل

Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors.

Human embryonic stem cells (hESCs) could potentially represent an alternative source for blood transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. When we cultured hESCs on either C3H10T1/2 or OP-9 cells to facilitate hematopoiesis, we found that exogenous administration of vascular endothelial growth factor promoted the emergence of sac-like structures, which...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2009